The Protein A ELISA Kit is a Sandwich ELISA for the highly sensitive detection and/or quantification of Protein A in fluids. The kit is designed to measure trace contamination of Protein A in antibody solutions. The antibodies used in this kit were produced by immunization with recombinant Protein A. Native and recombinant Protein A are quantified in this assay with similar potency.
Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987). Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sj