Protein A is a surface protein (approximately 56kD in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification.
This product was prepared from chromatographically pure Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein A.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1:10,000-1:40,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.