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Protein G (Biotin)

Cat no: 168452

Supplier: United States Biological
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This product was prepared from chromatographically pure Protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein G. No reaction was observed against anti-Protein A. Source: Pure protein G Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20,000-1:200,000 Western Blot: 1:10,000-1:40,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 168452
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 500ug
Form: Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with Biotin.
Purity: Purified by Protein G affinity chromatography.

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