For fast and simple integration of FRT-sites into large vector plasmids at any intended position (for generation of transgenic mouse models). The FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells. The included pCI-FLPe expression plasmid is coding for an improved FLP recombinase which shows a four to tenfold higher recombinational activity compared to the wildtype FLP enzyme at temperatures between 37Degree C (optimal growth temperature for E.coli) and 40Degree C (mouse body temperature). This kit can also be used to work on bacterial artificial chromosomes. High Red/ET efficiency plus convenient removal of the Red/ET Recombination protein expression plasmid pRedET after recombination.