For fast knockouts or modifications of genes on the E.coli chromosome. Red/ET recombination allows the exchange of genetic information in a base pair precise, specific, and faithful manner. An FRT-flanked kanamycin resistance marker cassette is supplied with the kit which can be used to replace a gene on the E.coli chromosome. Red/ET recombination can replace fragments as large as 30kb from the E.coli chromosome.
The use of a FRT-flanked resistance cassette for the replacement of the targeted gene allows the subsequent removal of the selection marker by a FLP-recombinase step, if required. FLP expression plasmids can be purchased.