Home  >  Products  >  RAF1, Active, Recombinant, Human (raf-1, raf 1, Active RAF1, RAF1, RAF, human RAF, active kinase, active kinases, recombinant RAF1, recombinant RAF, kinase activity assay, protein kinase)
RAF1, Active, Recombinant, Human (raf-1, raf 1, Active RAF1, RAF1, RAF, human RAF, active kinase, active kinases, recombinant RAF1, recombinant RAF, kinase activity assay, protein kinase)

RAF1, Active, Recombinant, Human (raf-1, raf 1, Active RAF1, RAF1, RAF, human RAF, active kinase, active kinases, recombinant RAF1, recombinant RAF, kinase activity assay, protein kinase)

Cat no: 167952

Supplier: United States Biological
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RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Source: Recombinant protein corresponding to human RAF1 expressed in Sf9 insect cells. Molecular Weight: ~63.0kD Biological Activity: The specific activity of RAF1(EE) was determined to be ~6000 nmol/min/mg in a coupled assay as per activity assay protocol. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: 167952
Size: 5ug
Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Purity: ~85% (SDS-PAGE)
References: 1.Rapp, U. et al: Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Nat. Acad. Sci. 80: 4218-4222, 1983. 2. Li, P. et al: Raf-1: a kinase currently without a cause but not lacking in effects. Cell 64: 479-482, 1991.

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