This is a new version of counter-selection cassette pRpsL-neo for the Red/ET Recombination system. It is based on Streptomycin selection. The kit is designed to modify any type of bacterial artificial chromosomes (BACs) within 2-3 weeks by using a counter-selection cassette. The included counter-selection cassette pRpsL-neo is based on Streptomycin selection which shows a much higher efficiency than pSacB-neo or comparable systems.
This kit can also be used to work on bacterial chromosomes and common ColE1 origin plasmids. This Kit combines the high Red/ET efficiency plus convenient removal of the Red/ET Recombination protein expression plasmid pRedET after recombination.