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Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Industry price, requires MTA)

Cat no: A202


Supplier: B-Bridge International, Inc.
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pCAGGS-FLPe-IRESpuro is an improved vector for expression of an enhanced FLP recombinase in mouse ES cells and oocytes. FLPe shows an enhanced thermo stability at 37Degree C compared to the normal FLP recombinase. After micro-injection of pCAGGS-FLPe into oocytes approximately one-third of heterozygotic mice born show complete FLP recombination. pCAGGS-FLPe-IRESpuro carries a strong, constitutive expression cassette for an in vitro-evolved FLP recombinase under the control of the chicken-s-actin promoter and an hCMV immediate early enhancer. The use of the chimeric CMV enhancer/s-actin promoter leads to a ubiquitous expression profile in eukaryotes.
Catalogue number: A202
Size: 0.2ug/ul,20ul

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