The loxP-PGK-gb2-neo-loxP template is designed to allow neomycin/kanamycin selection in prokaryotic and eukaryotic cells.
The loxP-PGK-gb2-neo-loxP template encodes the neomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells.