The loxP-FRT-PGK-gb2-neo-FRT template is designed to allow kanamycin/neomycin selection in prokaryotic and eukaryotic cells, respectively.
It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells.