High Resolution Melt (HRM) curve analysis is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature. It uses intercalating dyes, highly accurate melt curves and the application of specific statistical analyses. SensiFAST HRM has been developed using the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis. The combination of our SensiFAST DNA Polymerase, buffer and EvaGreen® dye enables amplification and discrimination of even the most challenging sequence differences, such as class 4 SNPs (Fig. 1) without sequence preference. Main applications of HRM include Single Nucleotide Polymorphisms (SNPs) genotyping, epigenetics (DNA methylation analysis), zygosity testing (DNA mapping and DNA fingerprinting) and gene scanning (search for the presence of unknown variations in PCR amplicons).
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