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Strep-tag(R) Starter Kit

Strep-tag(R) Starter Kit

Cat no: 2-1101-000


Supplier: IBA GmbH
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The Strep-tag(R) Starter Kit contains all essential reagents required for expression in E. coli, purification and detection of Strep-tag(R) proteins; sufficient for 8 applications.
Catalogue number: 2-1101-000
Research area: The (Twin-) Strep-tag(R)/Strep-Tactin(R) protein purification system provides the reliable one-step purification of highly pure proteins suitable for any application, including: Structural and functional investigations Crystallization for determination of 3D structure Due to the mild conditions Strep-tag(R) II recombinant proteins can also be used for: Assays involving protein-protein and protein-DNA interactions Investigating ligand-receptor interactions under physiological conditions Separating living cells for re-culturing purposes
Additional info: The kit contains: 1 ready-to-use column with Strep-Tactin(R) Sepharose(R), 1 ml, gravity flow Control plasmid with 15 kD protein insert Anhydrotetracycline for induction of expression Fractionation buffer for the preparation of a periplasmic extract Washing buffer for column chromatography or for the preparation of a cytoplasmic extract Elution buffer for displacing the Strep-tag(R) protein from the column Column regeneration buffer (with HABA) Strep-Tactin(R) horse radish peroxidase (HRP) conjugate for Western blot detection Comprehensive manual
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