Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.
Sample Type:
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids.
Intended Use:
Kit contains the necessary reagents for convenient measurement of activity of Superoxide Dismutase (SOD) by colorimetric method.
Test Principle:
The sensitive SOD BioAssay(TM) kit utilizes WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD (below). Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
Kit Components:
WST Solution, 1x1ml
SOD Enzyme Solution, 1x20ul
SOD Assay Buffer, 1x20ml
SOD Dilution Buffer, 1x10ml
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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