Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.
Intended Use:
Surfactant Pulmonary Associated Protein D, BioAssay(TM) ELISA Kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Surfactant Protein-D (SP-D). Kit is designed for the analysis of cell culture supernates. Other sample types, such as serum and plasma, need to be validated prior to use.
Each kit contains sufficient materials to run ELISAs on ~15x96-well plates, provided that the following conditions are met: The assay is run as summarized in the General ELISA protocol. The recommended microplates, buffers, diluents, substrates and solutions are used.
Range:
~156-10,000pg/ml
Kit Components:
Capture Antibody, 1x1vial
Detection Antibody, 1x1vial
Standard, 1x3vials
Streptavidin-HRP, 1x1vial
Note: Plates not included in Development Kit
Storage and Stability:
Store components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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