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Thioredoxin Reductase Colorimetric Assay Kit

Thioredoxin Reductase Colorimetric Assay Kit

Cat no: 10007892


Supplier: Cayman Chemical Company
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Cayman's TrxR Assay provides a convenient method for quantifying mammalian TrxR activity from tissue homogenates and cell lysates in a colorimetric 96-well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.
Catalogue number: 10007892
Weight: 0
Form: 96 Well
P type: Assay Kits
Shipping temp: -20
Storage temp: -20
Additional info: Cayman's Thioredoxin Reductase (TrxR) Assay Kit provides a convenient method for quantifying mammalian TrxR activity from tissue homogenates and cell lysates in a 96-well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm. The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.

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