TIMP-1, Mouse, BioAssay(TM) ELISA Kit (For Lysates)

Mouse TIMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) BioAssay(TM) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP-1. This assay employs an antibody specific for mouse TIMP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-1 antibody is added. After washing away unbound biotinylated antibody, HRP- conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The minimum detectable dose of TIMP-1 is typically less than 3pg/ml. Source: Mouse Sample Type: Cell and tissue lysates. Intended Use: The BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results. The antibody pair provided in this kit recognizes mouse TIMP-1. Sensitivity: The intra-Assay reproducibility is CV<10% and inter-Assay is CV<12%. Kit Components: TIMP-1 Microplate (Item A), 1x96wells Wash Buffer Concentrate, 1x25ml Standards (Item C), 2x vials Sample Diluent Buffer (Item D), 1x10ml Assay Diluent (Item E), 1x15ml Detection Antibody TIMP-1 (Item F), 2x vials HRP-Streptavidin Concentrate (Item G), 1x200ul TMB One-Step Substrate Reagent (Item H), 1x12ml Stop Solution (Item I), 1x8ml Cell lysate buffer (Item J), 2x5ml Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.