Tumor Necrosis Factor-b (TNF-b) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-b is a 18.6kD protein containing 171aa residues.
Source:
Recombinant corresponding to human TNF-b expressed in E.coli.
Molecular Weight:
~24kD
Biological Activity:
The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ~0.05ng/ml, corresponding to a specific activity of ~2x10e7 units/mg.
Endotoxin:
~0.1ng/ug
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.