Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications. Total Carbohydrate Assay is a simple, sensitive and robust method of detecting virtually all carbohydrates. The assay is based on the phenol-sulfuric acid method. In Total Carbohydrate Assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural, which react with the Developer to form a chromogen that can be quantified by measuring the absorbance at 490nm. The Total Carbohydrate Assay can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.
Sample Type:
Adherent or suspension cells, animal and plant tissues, food products, fruit juices & other beverages.
Intended Use:
Measurement of total carbohydrate in various samples.
Kit Components:
Assay buffer, 1x25ml
Developer, 1x3ml
Standard (D-glucose, 2mg/ml), 1x0.2ml
Storage and Stability:
Store liquid at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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