Mouse TPO ELISA (Enzyme-Linked Immunosorbent Assay) BioAssay(TM) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TPO. This assay employs an antibody specific for mouse TPO coated on a 96-well plate. Standards and samples are pipetted into the wells and TPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The minimum detectable dose of TPO is typically less than 30pg/ml.
Source:
Mouse
Sample Type:
Serum, plasma and cell culture supernatants.
Intended Use:
The BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results. The antibody pair provided in this kit recognizes mouse TPO.
Sensitivity:
The intra-Assay reproducibility is CV<10% and inter-Assay is CV<12%.
Kit Components:
TPO Microplate (Item A), 1x96wells
Wash Buffer Concentrate, 1x25ml
Standards (Item C), 2x vials
Assay Diluent A (Item D), 1x30ml
Assay Diluent B (Item E), 1x15mL
Detection Antibody TPO (Item F), 2x vials
HRP-Streptavidin Concentrate (Item G), 1x200ul
TMB One-Step Substrate Reagent (Item H), 1x12ml
Stop Solution (Item I), 1x8ml
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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