Transferrin Receptor, soluble, Human BioAssay(TM) ELISA Kit (sTfR)

The Soluble Transferrin Receptor (sTfR) has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia (IDA) and anemia of chronic disea se (ACD). The circulating sTfR concentration is proportional to cellular expression of the membrane-associated TfR and increases with increased cellular iron needs and cellular proliferation. Furthermore, because serum ferretin reflects the storage iron compartment and sTfR reflects the functional iron compartment, the sTfR/log ferritin index (sTfR-F-index), based on these two values, and has been suggested a s a good estimate of body iron compared with the sTfR/ferrin ratio. Distinguishing between IDA and ACD is a key step for determining whether iron supplementation would be beneficial. Intended Use: The quantitative determination of sTfR concentration in human serum or plasma by a microplate enzyme immunoassay Sensitivity: 0.055 nmol/l Specificity: 96% Range: 0-80 nmol/l Test Principle: The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies (enzyme and immobilized), with different and distinct epitope recongnition, in excess, and native antigen. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-sTfR antibody Kit Components: 1. sTfR ELISA test Calibrators: 0, 3.0,10, 20,40, & 80 nmol/L 2. sTfR EIA enzyme HRP conjugate reagent, 12 ml 3. sTfR EIA Biotin reagent, 12 ml 4. Streptavidin Coated Microplate 96 wells 5. sTfR ELISA test wash solution concentrate, 20ml 6. TMB substrate solution, 12 ml 7. Stop solution, 8 ml Storage and Stability: Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.