CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18kD soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant murine CD40 Ligand is a soluble 18kD protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.
Source:
Recombinant protein corresponding to mouse CD40Ligand/TRAP, expressed in E. coli.
Molecular Weight:
~16.4kD
Biological Activity:
ED50 is <0.1ug/ml determined by its ability to induce TNF-a and MIP-1a production by murine splenocytes.
Endotoxin:
<0.1ng/ug
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.