TRIsure is a ready-to-use reagent for the isolation of total RNA from cells and tissues. TRIsure maintains the integrity of the extracted RNA, while disrupting cells and subsequently dissolving cell components. After the addition of chloroform, the RNA can be recovered easily from the aqueous phase. DNA and protein can also be recovered after further precipitation steps. The RNA remains in the aqueous phase and is subsequently recovered by precipitation with isopropyl alcohol. The isolated RNA is suitable for any downstream application such as RT-PCR, hybridization assays, or in vitro translation. 1ml of TRIsure is sufficient to isolate total RNA from 1 x 107 cells or 100mg of tissue.
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