Urokinase (urokinase-type plasminogen activator or uPA, EC 3.4.21.73) is a serine protease, which catalyzes the conversion of the plasminogen to active plasmin, which in turn participates in thrombolysis.
Test Principle:
Urokinase Fluorimetric Activity BioAssay(TM) Kit utilizes an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450nm. This sensitive assay detects very low levels of Urokinase activity in biological samples (as low as 0.02 IU/ml).
Sample Type:
Serum, nuclear and cell extract.
Intended Use:
Applications include measuring Urokinase activity of purified enzymes and measuring Urokinase activity in serum and cell/nuclear extracts.
Kit Components:
Urokinase Assay Buffer, 1x25ml
Cell Lysis Buffer, 1x25ml
Urokinase Substrate, 1x0.20ml
Human Urokinase (Standard/Positive Control), 1x vial
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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