Urokinase (Urokinase-type plasminogen activator, uPA, EC 3.4.21.73) is a serine protease, which catalyzes the conversion of the plasminogen to active plasmin, which in turn participates in thrombolysis. Urokinase inhibitors have potential applications in cancer therapy.
Intended Use:
Applications include screening potential inhibitors of Urokinase and can used to characterize/study Urokinase inhibitors.
Test Principle:
Urokinase Inhibitor Screening BioAssay(TM) Kit utilizes the ability of Urokinase to cleave a synthetic AMC-based peptide substrate to release AMC, which can be detected by measuring its fluorescence at Ex/Em = 350/450nm. In the presence of the Urokinase specific inhibitors, the enzyme activity is inhibited or completely abolished. This assay kit is a simple, sensitive, and rapid tool to screen potential Urokinase inhibitors.
Kit Components:
Urokinase Assay Buffer, 1x25ml
Urokinase Substrate, 1x0.20ml
Human Urokinase, 1x vial
Urokinase Inhibitor (GGACK Dihydrochloride), 10mM, 1x10ul
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.