Vitamin B12 (VB12, Cyanocobalamin) BioAssay(TM) ELISA Kit

Vitamin B12 plays an important role in the functioning of a healthy body. It is essential to the production of red blood cells that carry oxygen through the body's tissue. This in turn helps in the development of myelination for proper functioning of the nervous system. The effects of Vitamin B12 deficiency can range from fatigue, weakness, depression, and memory problems to more severe symptoms such as anemia. Vitamin B12 deficiencies progress slowly, and thus it may take several years for initial signs to develop. Intended Use: Vitamin B12 ELISA Kit is an enzyme immunoassay (ELISA) for the quantitative detection of Vitamin B12 concentration in human serum. The Vitamin B12 ELISA test is for in- vitro diagnostic use only. Sensitivity: 70.13 pg/ml Test Principle: Delayed Competitive Enzyme Immunoassay (TYPE 9): The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen. Upon mixing the biotinylated antibody with a serum containing the antigen, a reaction results between the antigen and the antibody. After a short incubation, the enzyme conjugate is added (This delayed addition permits and increase in sensitivity for low concentration samples). Upon the addition of the enzyme conjugate, competition reaction results between the enzyme analogand the antigen in the sample for a limited number of antibody binging sites (not consumed in the first incubation). A simultaneous reaction between the biotin attachedto the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration. he enzyme activity in the antibody bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained. Kit Components: 1. Standard set for Vitamin B12 elisa test: 0, 100, 200, 400, 1000 & 2000 pg/ml 2. Vitamin B12 EIA enzyme conjugate reagent 3. Vitamin B12 EIA Biotin reagent 4. Streptavidin Coated Microplate 96 wells 5. Vitamin B12 EIA wash solution Concentrate, 20ml 6. TMB substrate, 12 ml 7. Stop solution, 8 ml 8. Vitamin B12 ELISA releasing agent, 12ml 9. Vitamin B12 ELISA stabilizing agent, 0.5 ml 10. Vitamin B12 ELISA neutralizing Buffer, 7 ml Storage and Stability: Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.