Interleukin 15 (IL-15, MGC9721)

Interleukin 15 (IL-15) is a cytokine that shares many biological activities with IL-2. Both cytokines interact with the b and g component of the IL-2 receptor. However, unlike IL-2 which is secreted only by T cells, IL-15 is expressed preferentially by nonlymphoid tissues, epithelial, and fibroblast cell lines (1). Two isoforms of human IL-15 are known. The longer form is localized to the plasma membrane or may be secreted, while the other (the shorter form) is found within the cytoplasm and the nucleus (2). IL-15 regulates T and natural killer cell activation and proliferation, causes B cell proliferation and differentiation and stimulates chemotaxis of T cells (3-4). It also induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. High concentrations of IL-15 have been observed in inflamed joints of rheumatoid arthritis patients, suggesting a role for IL-15 in inflammatory diseases where there is recruitment of leukocytes (5).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

I8437-10C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Giri JG, et al. EMBO J 13:2822, 1994 2. Gaggero, A., et al. 1999. Eur. J. Immunol. 29:1265. 3. Armitage RJ, et al. J Immunol 154:483, 1995 4. Wilkinson PC, et al. J Exp Med 181:1255, 1995 5. McInnes, IB, et al. 1998. Nat. Med. 2:175.

Additional Info

Recognizes human IL-15.