MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38 alpha, beta, gamma (also known as ERK6 or SAPK3) and delta (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8). \n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation:1:200 \nImmunofluorescence: 1:25\nFlow Cytometry: 1:25\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2352-05E

Size

200ul

Applications

ELISA, FC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Rouse, J. et al. (1994) Cell 78, 1027-1037. 2. Han, J. et al. (1994) Science 265, 808-811. 3. Lee, J.C. et al. (1994) Nature 372, 739-746. 4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049. 5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426. 6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534. 7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30. 8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.

Additional Info

Recognizes endogenous levels of human p38 MAP kinase only when dually phosphorylated \nat Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species Crossreactivity: mouse, rat, monkey and mink.

Alternative Names

EC=2.7.11.24