Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.ELISA and Western ELISA may be performed at 10-100ng protein depending on the nature and affinity of the detection reagent. HIV-1 converted human serum polyclonal antibodies yield titers of up to 1:100,000 at 10ng of immobilized protein under standard ELISA conditions.