A novel U2OS/tGFP-FPRL1 cell line has been developed through stable transfection to modulate the internalization of the Human Formyl peptide receptor 2 (FPRL1) complex in living cells and to assay for compounds inducing tGFP-FPRL1 redistribution inside the cells. Fluorescent FPRL1 Internalization Assay cell line was obtained by stable transfection of an expression vector for a fusion protein of tGFP and human Formyl peptide receptor 2, as well as G418 into U2OS cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively express the tGFP-FPRL1 fusion protein.Assay Details: Innoprot's FPRL1 Internalization Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate kFormyl peptide receptor 2 activation and the following redistribution proce Beta inside the cells. This highly reproducible a Betaay allows monitoring FPRL1 receptor activation and redistribution process in High Content Analysis and fluorescence microscope applications. This internalization assay was validated with an average of Z=0.70 +/- 0.02 for High Content Screening.
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