MAPKAPK2, Active, Recombinant, Human (MAPKAPK 2, MAPKAPK-2, Active MAPKAPK2, MAPKAPK2, MAPKAPK, human MAPKAPK2, active kinase, active kinases, recombinant MAPKAPK2, recombinant MAPKAPK, kinase activity assay, protein kinase)

MAPKAP kinase2 is a Ser/Thr protein kinase, which is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo.\n\nSource:\nRecombinant protein corresponding to human MAPKAP kinase 2 expressed in Sf9 insect cells.\n\nMolecular Weight: \n~68.0kD\n\nBiological Activity:\n254 nmol phosphate incorporated into MAPKAP kinase 2 substrate peptide per minute per mg protein at 30 degrees C for 15 minutes using a final concentration of 50um ATP and total of 0.83uCi/ul P-32.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167908

Size

5ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5,150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Stokoe, D.; Caudwell, B.; Cohen, P. T. W.; Cohen, P.: The substrate specificity and structure of\nmitogen-activated protein (MAP) kinase-activated protein kinase-2. Biochem. J. 296: 843-849, 1993.\n2. Maizels, E. T.; Mukherjee, A.; Sithanandam, G.; Peters, C. A.; Cottom, J.; Mayo, K. E.; Hunzicker- Dunn, M.: Developmental regulation of mitogen-activated protein kinase-activated kinases-2 and -3 (MAPKAPK-2/-3) in vivo during corpus luteum formation in the rat. Molec. Endocr. 15: 716-733, 2001.\n3. McCormick, C.; Ganem, D.: The kaposin B protein of KSHV activates the p38/MK2 pathway and stabilizes cytokine mRNAs. Science 307: 739-741, 2005.