Ribonuclease H from E. coli.
Ribonuclease H is an endonuclease which specifically degrades the RNA moiety of a DNA:RNA hybrid, without affecting the unhybridized RNA. It does not affect double-stranded RNA or DNA.
It is provided in a 50% glycerol storage buffer.
Applications: Enabling the synthesis of second-strand cDNA by removal of the RNA; Used in conjunction with AMV RT and T7 RNA Polymerase in NASBA amplification of RNA.