Nucleic Acid Analysis
Nucleic acids, the large macromolecules that make up DNA, RNA and proteins, are essential to the existence of all living organisms and non-living entities such as bacteria and viruses. Nucleic acids are made up of chains of nucleotides, each of which contains a base, a sugar and a phosphate group. The specific arrangement of these nucleotides forms a double stranded helix of DNA, a single-stranded molecule of RNA, or a 3-dimensional protein molecule.
Nucleic acid analysis includes DNA sequencing (determining the order of nucleotide bases in a DNA molecule); DNA synthesis (creating DNA fragments to use as microarray probes and for gene cloning, sequencing and amplification); and genotyping (analysis of how genes are expressed, for example, in diseased vs. normal tissues). Applications of nucleic acid analysis are found in genomics, proteomics, pharmaceuticals, clinical diagnostics, agriculture, forensics, and biotechnology.
Instruments for nucleic acid analysis include real-time nucleic acid analysis systems; spectrometers (including Raman); microscopes; spectrophotometers; microspectrophotometers; luminometers; multimode microplate readers; spectroscopy systems; chromatography systems (including HPLC); particle analyzers; capillary electrophoresis systems; and chemiluminescent systems.
Equipment for nucleic acid analysis includes pH, ion and conductivity meters; tissue homogenizers; centrifuges; sample prep coolers; thermal cyclers; LIMS and DNA and protein sequencing software; gel and blot imaging systems; LC column heaters and chillers; autosamplers; microplate washers and stackers; OEM liquid and plate handling robotics; and hybridization stations.
Kits and consumables for nucleic acid analysis include DNA and RNA purification kits; DNA collection, storage, stabilization and transportation kits; micro RNA PCR kits; DNA fragment library prep kits; blocking solution; lysing kits; transfection reagents; probes; adsorbents; detection reagents; staining kits; cuvettes; growth containers; cell culture media; endotoxin detection assays; and DNA sequencing services.
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