Microarray Technology
Microarray technology is a method of greatly increasing the throughput of laboratory processes to efficiently perform large-scale genetic tests, such as comparing gene expression between healthy and tumorous tissues. DNA microarray technology is being used for research on heart disease, mental illness and infectious diseases, as well as for developing targeted cancer treatment strategies.
Performing a microarray analysis requires a machine that can automatically dispense tens of thousands of microscopic dots of DNA fragments in a regular matrix, or array, on the surface of a slide. The slide is called a DNA chip and the deposited DNA spots are called probes, or oligos. The probes are covalently bound to the surface of the slide, or synthesized directly on the slide, and a computer keeps track of which probe is located at each position. Alternatively, the probes are bound to microscopic polystyrene beads.
To prepare the target, DNA samples are purified, treated with reverse transcriptase (RT) enzyme to generate complementary DNA (cDNA), and then coupled with fluorescent or chemiluminescent dye molecules, to allow test results to be read. To perform the microarray analysis, the target cDNA is “hybridized” to the DNA probes on the chip with hydrogen bonds that naturally form between complementary nucleic acid sequences on the target and the probes. The more complementarity, the more strongly the target will bind to each specific probe on the chip.
The relative amounts of cDNA that have been bound to each probe can be “read” by a machine scanning the chip with a laser to illuminate the dye molecules, and then processing the image to detect the presence and strength of dye found at each spot.
Equipment and supplies used for microarray technology includes homogenizers, heating and cooling blocks, and mini-centrifuges to prepare the target; DNA, RNA and protein extraction kits; automated nucleic acid purification equipment; PCR systems to amplify the target; and microscopes and spectroscopes to read the test results.
Browse the product range below or simply use the Search Tool above to help find the best products.
Once you have found a product, complete a ‘Get a Quote’ form and you will get the best price!
Showing 24 of 26 products
Product A-Z
Showing 24 of 26 products
Latest promotions
Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...
Genovis introduces GlycINATOR™• Works on most IgGs• Effective on high-mannose and bisected N-linked Fc-glycans • Rapid – 30-minute...
Use promo code EASY3 to receive 13% off and FREE shipping!Experience a new dimension of electronic pipetting with the NEW Easypet 3 pipette controller. The...
New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...
As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....
It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...
Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...
We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!
For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...
10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...
Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...
Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...
Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...
Top suppliers
Carl Zeiss Microscopy
27 products
Eppendorf
1 products
Promega Corporation
11 products
Panasonic Healthcare Company
5 products
Life Technologies
1 products
Nikon Instruments Europe
11 products
Olympus Europa Holding GmbH
3 products
Leica Microsystems, Inc.
10 products
GE Healthcare Life Sciences
2 products
Tecan Trading AG
19 products
Beckman Coulter, Inc.
1 products
United States Biological
230753 products
AB SCIEX
3 products
BD (Becton, Dickinson and Company)
1 products
RANDOX TOXICOLOGY
5 products
Randox Food Diagnostics
6 products