Plant Biology
Using RNAi technology, which silences genes that develop certain proteins, plant biologists have developed varieties that are resistant to viruses, such as Cucumber and Tobacco Mosaic Virus, Tomato Spotted Wilt Virus, and others. Plants have also been genetically modified to resist insect pests and parasitic nematodes, such as root-knot nematode and bollworm. RNAi, gene cloning and...
...transfection, and other methods have enabled the enhancement of nutrients such as lycopene and beta carotene; reduction of caffeine in coffee and nicotine in tobacco; delayed ripening and improved shelf-life of tomatoes; virus resistance of potatoes, squash and papayas; herbicide tolerance of rice, alfalfa and canola; and creation of a “tearless” onion.
In addition to agricultural R&D, plant biology labs also contribute to pharmaceutical research, clinical testing, and environmental monitoring, as well as verification, safety and quality assurance in the drug, beverage and cosmetic industries.
Plant biology laboratory instrumentation includes in vivo plant imaging systems; microplate readers; gamma counters; radio flow detectors; liquid, ion and HPLC chromatography systems; luminometers; fluorometers; spectrophotometers; microscope systems and HD microscopy cameras.
Equipment and supplies for plant biology labs include biological stirrers; roller bottle systems; incubators; shakers; coolers and thermal cyclers; mills and homogenizers; cryogenic freezers; laminar flow cabinets; colony counters; and sequencing and analysis software; cryogenic tubes; adsorbents; cell culture media; plant culture containers and cell freezing containers.
Biochemicals and reagents for plant biology include assay kits; DNA, RNA and protein extraction kits; mycoplasma removal agent; cell lysing kits; transfection reagents; restriction enzymes for gene knock-out or gene modification; primary and secondary antibodies for plant biology, including anti-fucose and anti-xylose; the argonaute protein, a catalyst for the RNA-induced silencing complex (RISC); plasma membrane proteins that mediate metabolic uptake and movement of plant leaves; and a catalyst for CO2 fixation in photosynthesis.
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