RNAi Technologies
RNAi technologies are based on metabolic pathways of gene suppression, or silencing, through the binding of either micro RNA (miRNA) or small “interfering” RNA (siRNA) to specific messenger RNA (mRNA) molecules. The presence of these small molecules prevents the mRNA from producing a protein.
Researchers use this mechanism of RNA interference (RNAi), and the results of genome sequencing, to identify individual gene functions and to screen for therapeutic drug candidates that may be able to interfere with the production of disease-causing proteins. Thus, RNAi technology may help doctors treat cancer, heart disease, neurodegenerative disease and others. Agricultural researchers have already put RNAi technology to good use, developing crops with higher yields, higher nutrient levels, and resistance to viruses, nematodes and insects, as well as completely new crops such as non-allergenic peanuts.
Lab instruments and equipment for RNAi technologies include microplate readers; cell culture and analysis systems; real-time PCR systems; ultra-low freezers; centrifuges; tissue homogenizers; microvolume cuvettes; sample prep cooling systems; and biobanking software.
Biochemicals and reagents for RNAi technologies include custom oligos, DNA and RNA; lentiviral constructs; TALE DNA-binding and nuclease-fused reagents for gene knockout and gene modification; shRNA library modules; micro RNA PCR kits; RNAi screens; cell lysing kits; RNA extraction kits; DNA sequencing services; and qPCR primers and assays.
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