Description
AMSBIO offers a proven service for synthesis of custom-designed, long mRNA and highly modified mRNAs. Working from customer supplied target sequence data - AMSBIO has the experience, expertise and facilities to provide a complete, high quality mRNA custom design service. Synthetic mRNA from AMSBIO is supplied highly purified and ready-to-use.Following chemical synthesis of the synthetic DNA, the dsDNA fragments are cloned in a plasmid, purified and full sequencing documentation produced. PCR amplification of the cloned inserts with the primers is then undertaken, purified using spin columns and the resultant DNA template characterised by capillary electrophoresis. The subsequent RNA synthesis uses proven in vitro transcription methodologies and the resultant custom designed mRNA product is then highly purified and fully characterised ready for your use.
While long mRNA can be used as a standard, internal control and for in vitro diagnostics, synthetic modified mRNA is the most advanced and safest method for deriving iPS cells. AMSBIO now offers a non-integrating strategy for reprogramming stem cell fate based on the administration of synthetic modified mRNA that greatly increases efficiency whilst reducing the innate antiviral response of viral protocols.
