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1,3-Butadiene, Conjugated

Cat no: B8997-10

1,3-Butadiene, Conjugated

Applications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nSpecificity:\nUsing a conjugate 1,3-Butadiene, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:\n\nCompound Crossreactivity ratio(a)\nButadiene-BSA 1 \nAcrolein-BSA 1/20,000\nFormaldehyde-BSA 1/>50,000\nMalondialdehyde-BSA 1/>50,000 \nButanoic acid-BSA 1/>50,000\nPropionic acid-BSA 1/>50,000\n\n(a): 1,3-Butadiene-BSA concentration/conjugated close structure concnetration at half displacement.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 2 months at 4 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B8997-10

Size

100ug

Applications

ELISA

Hosts

Rabbit

Form

Supplied as a lyophilized powder. Reconstitute with 100ul sterile dH2O.

P Type

Pab

Purity

Purified by protein carrier (BSA) affinity column, and ammonium sulphate precipitation.

Isotype

IgG

Additional Info

Recognizes 1,3-Butadiene conjugated with BSA. See additional crossreactivity data.

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