14-3-3, beta, zeta

14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins \n(1,2). 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain (3). The 14-3-3 proteins exists in vitro and in vivo as either homo-or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C (4). 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors (5).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot (ECL): 1ug/ml\nImmunohistochemistry (PC): 1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

0014-03B

Size

25ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Aitken, et al., Biochem. Soc. Trans. 23: 605

Additional Info

Reacts specifically with two members of the 14-3-3 protein family: the acidic human IEF58 (~29kD) and IEF57a (~30kD), which correspond to 14-3-3 beta and zeta, respectively. Recognizes epitopes on the cytoplasmic face of the Golgi membrane. \nSpecies Crossreactivity: human, mouse, rat, monkey, rabbit, canine, sheep, porcine and bovine.