14-3-3, gamma (Protein kinase C inhibitor protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG)

14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. \n14-3-3 proteins form homo- and hetero-dimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. \n\nApplications: \nSuitable for use in Immunoblot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoblot: \n0.05-0.1mg/ml detects 14-3-3 gamma in RIPA lysate from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-14-3-3 gamma (0.05mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.\n\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: F4209\n3T3/A31 cell lysate. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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