14-3-3, pan

14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. \n\nApplications:\nSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunoprecipitation (Denatured): 2ug/mg protein lysate using Protein G.\nWestern Blot: 1-2ug/ml for 2 hours at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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