14-3-3, phosphorylated (Ser58)

Applications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

0014-01P

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10mM HEPES, pH7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Dougherty, M.K. et al. (2004) Unlocking the code of 1433. J. Cell Science. 117: 18751884. 2. Woodcock, J.M. et al. (2003) The dimeric versus monomeric status of 1433zeta is controlled by phosphorylation of Ser58 at the dimer interface. J. Biol. Chem. 278: 3632336327. 3. Yang, H. et al. (2006) DNA damage induced Protein 1433 (sigma) inhibits Protein Kinase B/Akt activation and\nsuppresses Aktactivated cancer. Cancer Res 66: 30963105.

Additional Info

Recognizes rat 1433 at ~29kD when phosphorylated at Ser58. Species Crossreactivity: human. Species sequence homology: zebrafish, mouse, dog, xenopus, chicken, sheep, bovine, monkey.

Alternative Names

Protein Kinase C Inhibitor Protein 1, KCIP-1, KCIP 1, Protein 1054