14-3-3 zeta (14-3-3 Protein zeta/delta, Protein Kinase C Inhibitor Protein 1, KCIP-1, YWHAZ)

14-3-3 zeta is a 245aa containing protein belonging to the 14-3-3 family mediating signal transduction by binding to phosphoserine containing proteins. It is a highly conserved protein in both plants and mammals. The encoded protein interacts with IRS1 implicating a plausible role in regulating insulin sensitivity. There are several transcript variants, with seven known mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta and eta. 14-3-3 proteins function in broad regulation of proteins by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, and activation/repression of enzyme activity. The protein is highly abundant in brain.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 3-5ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBrain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

0014-22H

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Bov, Can, Prc

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Murthi, P. et al. Placenta 29:798-801 (2008). 2. Frasor, J. et al. Cancer Res. 66:7334-7340 (2006). 3. Heidenblad, M. et al. BMC Med. Genomics 31:3 (2008).

Additional Info

Recognizes human 14-3-3 zeta. Species Crossreactivity: chimpanzee, bovine, canine, mouse, opossum, porcine. Species sequence homology: xenopus.