17 alpha Hydroxyprogesterone, Human, BioAssay(TM) ELISA Kit

The steroid 17 alpha-OH Progesterone is produced by the adrenal cortex and gonads. The 17 alpha-OH Progesterone has little progestational activity, but it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Cpd-S is produced by 21- hydroxylation of 17 OHP, measurement of 17aOHP is a useful indirect indicator of 21-hydroxlase activity. In congenital 21-hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17 a OHP is secreted in abundant excess. Measurement of 17 alpha-OHP is therefore valuable in the initial diagnosis of CAH.\n\nSample Type:\nSerum. Other fluids not optimized.\n\nIntended Use:\nFor quantitative determination of 17 alpha Hydroxyprogesterone in human serum.\n\nSensitivity:\n0.11ng/ml; 75 min assay;\n\nRange:\n- Newborn\n5-30 day: 0.7-2.5\n31-60 day male: 0.8-5.0\n31-60 day female: 0.5-2.3\n- Children: \n 3-14 yrs: 0.07-1.7\n- Females\n Follicular phase: 0.2-1.3\n Luteal phase: 1.0-4.5\n Postmenopausal: 0.2-0.9\n\nTest Principle:\nThe principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of 17alpha-OHP in the sample. A set of standards is used to plot a standard curve from which the amount of 17alpha-OHP in patient samples and controls can be directly read\n\nKit Components:\nT9006-04T1: Microwell Strip Plate: 1x96 wells\nT9006-04T2: Standard,0ng/ml: 1x2ml\nT9006-04T3: Standard, 0.15ng/ml: 1x600ul\nT9006-04T4: Standard, 0.5ng/ml: 1x600ul\nT9006-04T5: Standard, 1.5ng/ml: 1x600ul\nT9006-04T6: Standard, 3ng/ml: 1x600ul\nT9006-04T7: Standard, 7.5ng/ml: 1x600ul\nT9006-04T8: Standard, 20ng/ml: 1x600ul\nT9006-04T9: Control 1: 1x600ul \nT9006-04T10: Control 2: 1x600ul\nT9006-04T11: 17-OHP (HRP), 100x: 1x300ul \nT9006-04T12: Assay Buffer: 1x20ml \nT9006-04T13: Wash Buffer, 10x: 1x50 ml \nT9006-04T14: TMB Solution: 1x16ml\nT9006-04T15: Stop Solution, H2SO4: 1x6ml\n\nStorage and Stability:\nStore liquids at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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