Lambda Max:
262nm
Quality Control:
Tested in PCR with Taq DNA. Production of 1000bp PCR fragment from 2ng genomic DNA.
Applications:
Suitable for use in PCR, RT-PCR, cDNA, synthesis and primer extension. Other applications not tested.
Recommended Dilutions:
Optimal dilutions to be determined by the researcher.
Extinction Coefficient: 10x10e3 (pH 7.0)
Storage and Stability:
Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Endo- and Exonucleases:
Incubation of single stranded and double stranded radiolabeled oligonucleotides with 1ul of 20mM dUTP for 4 hours at 37 degrees C, separation on denaturing polyacrylamide gel and phosphoimaging did not detect DNA degradation.
Ribonucleases:
Incubation of 2000 base RNA transcript with 1ul of 20mM dUTP at 37 degrees C for 4 hours and separation on agarose gel resulted in no decrease in RNA transcript band intensity compared to control.
Nicking Activities:
Incubation of 1ug of supercoiled pUC19 DNA with 1ul of 20mM dUTP at 37 degrees C for 17 hours and separation on agarose gel did not generate linearised plasmid, and relaxation of supercoiled plasmid as compared to control.
E.coli DNA:
Quantitative PCR test on ABI Prism 7000 SDS, which uses amplification of E.coli 23S rRNA gene fragment did not detect E.coli DNA.
Human DNA:
Quantitative PCR test on ABI Prism 7000 SDS, which uses amplification of human genomic DNA fragment did not detect human DNA.
Functional Test:
PCR amplification of a 354 bp fragment of human apoprotein gene from 50pg human genomic DNA using Hot Start Taq DNA Polymerase.
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