Supplier:

United States Biological

Cat no: D3206-05

2

Lambda Max: \n262nm\n\nQuality Control: \nTested in PCR with Taq DNA. Production of 1000bp PCR fragment from 2ng genomic DNA.\n\nApplications:\nSuitable for use in PCR, RT-PCR, cDNA, synthesis and primer extension. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nExtinction Coefficient: 10x10e3 (pH 7.0)\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nEndo- and Exonucleases:\nIncubation of single stranded and double stranded radiolabeled oligonucleotides with 1ul of 20mM dUTP for 4 hours at 37 degrees C, separation on denaturing polyacrylamide gel and phosphoimaging did not detect DNA degradation. \n\nRibonucleases: \nIncubation of 2000 base RNA transcript with 1ul of 20mM dUTP at 37 degrees C for 4 hours and separation on agarose gel resulted in no decrease in RNA transcript band intensity compared to control. \n\nNicking Activities: \nIncubation of 1ug of supercoiled pUC19 DNA with 1ul of 20mM dUTP at 37 degrees C for 17 hours and separation on agarose gel did not generate linearised plasmid, and relaxation of supercoiled plasmid as compared to control. \n\nE.coli DNA:\nQuantitative PCR test on ABI Prism 7000 SDS, which uses amplification of E.coli 23S rRNA gene fragment did not detect E.coli DNA. \n\nHuman DNA: \nQuantitative PCR test on ABI Prism 7000 SDS, which uses amplification of human genomic DNA fragment did not detect human DNA. \n\nFunctional Test: \nPCR amplification of a 354 bp fragment of human apoprotein gene from 50pg human genomic DNA using Hot Start Taq DNA Polymerase.

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SPECIFICATIONS

Catalog Number

D3206-05

Size

100mM

Form

Supplied as a liquid in 100mM aqueous solution, pH 7.0.

Purity

(same/more than) 99% (HPLC)

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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