Two-dimensional difference gel electrophoresis (2D-DIGE) is a novel quantitative proteomics technique that evolved from traditional two-dimensional gel electrophoresis (2-DE). The principle of 2D-DIGE for separating mixed proteins is the same as traditional 2-DE. It uses differences in protein isoelectric points and molecular weights to separate protein mixtures. At the same time, the sensitive fluorescent dyes and the internal standard make it significantly better than traditional 2-DE in quantitativ......
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