3-Hydroxykynurenine (3-OHKYN)

3-hydroxykynurenine, a chromophore metabolite of tryptophan, acts as UV protectant and an antioxidant in vertebrate lens. This compound reacts with proteins to induce cross-linking and chromophore adducts.\n\nApplications: \nSuitable for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (paraffin) and Western Blot, Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry/Immunofluorescence: 1:100-1:200\nWestern Blot: 2ug/ml. Band at ~66kD observed for 3-OHKYN-modifed BSA.\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization:\nCytoplasmic\n\nPositive Control:\n3-OHKYN modified BSA\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H9100

Size

100ul

Applications

ELISA, ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquidin Tris-glycine, 150mM sodium chloride, 0.05% sodium azide

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG1,k

References

Staniszewska, MM and Nagaraj, RH. 3-hydroxykynuenine-mediated modification of human lens proteins. JBC. 280 (23): 22154-22164, 2005

Additional Info

Specific for 3-hydroxykynurenine modifications on N-alpha-acetyl lysine, N-alpha-acetyl histidine, N-alpha-acetyl arginine, and N-alpha-acetyl cysteine. It does not recognize the transaminase enzyme involved in the degradation of tryptophan. Reacts with 3-OHKYN modifications in human proteins.