4-1BB was originally described as a cDNA expressed by activated murine T cells and subsequently demonstrated to encode a member of the tumor necrosis factor receptor family of integral membrane proteins. Recently, a murine ligand for 4-1BB (mu4-1BB-L) was cloned
and demonstrated to be a member of an emerging family of ligands with structural homology to tumor necrosis factor. Both monoclonal antibody to hu4-1BB and cells transfected
with hu4-1BB-L induced a strong proliferative response in mitogen co-stimulated primary T cells. In contrast, ligation of 4-1BB on T cell clones enhanced activation-induced cell death when triggered by engagement of the TCR/CD3 complex. (1). To reject tumors, T cells must overcome poor tumor immunogenicity and an adverse tumor microenvironment. It has been shown that primary human T cells expressing CD80 and 4-1BBL vigorously respond to tumor cells lacking costimulatory ligands and provoke potent rejection of large, systemic tumors in immunodeficient mice.
Applications:
Suitable for use in Western Blot, Immunohistochemistry , Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:40
Optimal dilution determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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