4-1BB (TNFRSF9, tumor necrosis factor receptor superfamily, member 9, 4-1BB, 4-1BB ligand receptor, CD137, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9) (PE)

4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4-1BB cDNA encodes a 256aa residue type I transmembrane protein.1-3 A soluble 4-1BB is released from the surfaces of cells expressing the transmembrane protein.4 Mouse 4-1BB shares approximately 60% aa sequence identity with its human counterpart. 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, dendritic cells (DCs), and eosinophils.5 The ligand for 4-1BB (4-1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. In vivo, the co-stimulatory activity of 4-1BB has been shown to be important in graft-vs-host disease and antiviral CTL responses. On dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and up-regulates expression of B7-1 and B7-2 co-stimulatory molecules, resulting in an improved ability to stimulate T cell responses.1-5.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMyeloma cells with B cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

0004-01X

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% sodium azide, 0.5% BSA. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein A affinity chromatography.\n

Isotype

IgG2a

References

1. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631. 2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219. 3. Kwon, B.S. & S.M. Weissman (1989) Proc. Nat. Acad. Sci. USA 86:1963. 4. Wilcox, R.A. et al. (2002) J. Immunol. 168:4262. 5. Kwon, B. et al. (2002) Trends Immunol. 23:378.

Additional Info

Recognizes mouse 4-1BB/TNFRSF9.