4-Amino Biphenyl DNA (4-AMBP DNA, 4-Aminobiphenyl DNA)

4-amino Biphenyl (4-ABP) is an aromatic amine (arylamine). 4-ABP can be found as a constituent of tobacco smoke, in the form of an environmental carcinogen. It is metabolized by hepatic cytochrome P4501A2 to yield a direct-acting mutagen known as N-hydroxy-aminobiphenyl. Yet, the accumulation of biologically active metabolites is dependent on the activation and detoxification of N-acetylation. \n\n4-ABP has been shown to be a bladder carcinogen in humans and rodents. As well as genetic factors, 4-ABP may also play a role in breast cancer. It is hypothesized that smokers may have higher levels of DNA adducts than non-smokers and that the role of 4-ABP-DNA adducts and their relationship to tobacco exposure may be related to breast tumorigenesis. The antibody has been used to investigate the role of 4-ABP in the study of human breast cancer by measuring 4-ABP-DNA adducts.\n\nCellular Localization: Nuclear\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:50-1:100\nImmunohistochemistry: 1:50-1:100\nImmunocytochemistry: 1:50-1:100\nImmunofluorescence: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1401

Size

100ul

Applications

ELISA, ICC, IF, IHC

Hosts

Mouse

Form

Supplied as a liquid (ascites).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

References

1. al-Atrash, J., et al, Quantitative immunohistochemical analysis of 4-aminobiphenyl DNA in cultured cells and mice: comparison to gas chromatography/mass spectroscopy analysis. Chem. Res. Toxicol. 8(5): 747-752(1995) [uses this clone] 2. McQueen, CA., et al, Neonatal ontogeny of murine arylamine N-acetyltransferases: implications for arylamine genotoxicity. Toxicol. Sci. 73: 279-286 (2003) [uses this clone] 3. Faraglia, B., et al, Evaluation of 4-aminobiphenyl-DNA adducts in human breast cancer: the influence of tobacco smoke. Carcinogenesis. 24(4): 719-725 (2003) [uses this clone] 4. Al-Atrash, J., et al. Quantitative immunohistochemical analysis of 4-aminobiphenyl DNA in cultured cells and mice: comparison to gas chromatography/mass spectroscopy analysis. Chem Res Toxicol. 8(5):747-52, 1995. 5. Brown, A., et al. Robust DNA Repair May Lower Breast Cancer Risk. Journal of the National Cancer Institute. 2005.

Additional Info

This antibody is specific for 4-amino Biphenyl DNA. Crossreactivity: All DNAs