4D-DIA quantitative proteomics is an emerging high-throughput mass spectrometry technology that combines data-independent acquisition (DIA) strategies with four-dimensional (4D) separation techniques. It involves digesting protein into peptide by protease and separating peptide by liquid chromatography (LC), followed by mass spectrometry (MS) for detection and identification. It enables high-throughput and high-sensitivity quantitative analysis of proteins in biological samples.
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